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In some instances, flexor digitorum brevis (FDB) muscles were used for live imaging of calcium fluxes in single intact fibers.
Cells were maintained for 24 h in culture medium containing 2% FBS and then fixed or used for live imaging (see FLAP experiments).
A time interval of 30 s was used for live imaging.
A GFP-NHS-1A (GFP-NHS) construct was used for live imaging in MCF7 cells.
The Hes1 promoter – ubiquitinated luciferase reporter used for live imaging was a generous gift from R. Kageyama (Kyoto University, Japan).
A Zeiss Axiovert 200 inverted phase-contrast microscope outfitted with epifluorescence was used for live imaging studies.
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The sequences cloned into the vector to knockdown Spire1C were 5′-TCGAGGGATTAGACGTAGCAGATTATTCAAGAG ATAATCTGCTACGTCTAATCTTTTTTACGCGTA-3′ (Spire1C 3′ UTR, used for fixed cell imaging) and 5′-TCGAGGCGAATAATCTCCTGACTAATTCAAGAGATTAGTCAGGAGATTATTCGTTTTTTACGCGTA-3′ (Spire1C ORF, used for live cell imaging).
Forty-eight hours after transfection the cells were used for live cell imaging.
All neurons used for live cell imaging were cultured in Mattek dishes.
It is also commonly used for live cell imaging and it is synthetically readily accessible.
Fission yeast cells were grown under the same conditions that were used for live cell imaging.
More suggestions(15)
used for retinal imaging
used for live action
used for diagnostic imaging
used for visual imaging
used for optical imaging
used for live cancer
used for thermal imaging
used for live voice
used for acoustic imaging
used for live performance
used for medical imaging
used for live music
used for parallel imaging
used for cellular imaging
used for microvascular imaging
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