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An endocytic mechanism has been shown to be used for internalization of E. coli into HBMEC [22].
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After three days of culture the cells were washed and used for internalization assay.
As determined by titration of peptide, 50 nM of H1299.3 fluorescence conjugate resulted in sufficient signal-to-noise ratio for detecting internalization by the flow cytometry assay and thus this concentration is used for subsequent internalization assays.
Therefore, only data from cells exposed to NPs in the presence of serum were used for quantification of particle internalization.
Since the underlying mechanism of inhibition of internalization is similar in phagocytes and HeLa cells, the term antiphagocytosis is hereafter used for defining inhibition of bacterial internalization by both cell types (the term does not include the virulence plasmid independent avoidance of intracellular killing [4]).
Transmission electron microscopy (TEM) was used for the study of NPs internalization by 3T3 Swiss albino mouse fibroblasts.
Likewise, controversies remain concerning the endocytotic pathway used for the cell internalization pathway of CPP−cargo conjugates with data advocating all three major routes (2, 3).
In this study, we showed that the capacity of pSn to mediate antibody-induced internalization can be used for specific targeting of toxins and antigens to macrophages.
Additionally, confocal laser scanning microscope (CLSM) is also used for observation the internalization and location of FITC-labeled NPs.
We provide a methodology for the isolation of mitochondria to allow for clinical relevance and we discuss the methods we and others have used for the uptake and internalization of mitochondria.
Corona proteins have dedicated cell surface receptors that can be used for the binding and internalization of protein NP complexes.
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