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The existing steel sting, with modular construction, has been used for initial analysis.
Fresh semen was used for initial analysis and plasma membrane integrity and sperm morphology were also determined.
ElDorado tool from Genomatix GmbH (http://www.genomatix.de/) was used for initial analysis of STK35 gene in human genome.
The first 1000 base pairs upstream of the given 5'-end were typically used for initial analysis.
To compare control, mutant and revertant lines, the Student's t-test was used for initial analysis of lifespan, locomotion, fecundity, viability, and transcript amount.
DAVID Resources v. 6.7 ([ 48, 49] http://david.abcc.ncifcrf.gov/) was used for initial analysis to identify pathways generated by functional annotation clustering of gene lists and visualize genes on KEGG pathway maps.
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The Robust Multi-array Analysis (RMA) algorithm was used for initial intensity analysis [ 21] (Additional file 4).
The best hit was used for initial phylogenetic analysis as described above.
The pGWB533 vector contains the Gateway® cassette upstream of the uidA coding region (GUS), resulting in promoter: GUS fusion constructs used for initial promoter analysis and tobacco transformations.
The most basic model, y ijkg = Tissue i + Breed j + Sex k + Probe g + e ijkg, was used for initial exploratory analysis.
A BD LSR II flow cytometer or a BD FACSAria II cell sorter (BD Biosciences) equipped with three excitation lasers (405 nm, 488 nm and 633 nm) was used for initial FCM analysis.
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