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Haploview program version 4.1 (www.broad.mit.edu/mpg/haploview/) was used for haplotype analysis and for pairwise linkage disequilibrium, D′ and r2.
Haploview program version 4.1 (http://www.broad.mit.edu/mpg/haploview/) was used for haplotype analysis and for pairwise linkage disequilibrium, D′ and r2.
DNA microsatellite markers were used for haplotype analysis.
Sequence information around the insertion site yields allelic information and can be used for haplotype analysis.
The programme PLINK v1.07 was used for haplotype analysis (Purcell et al., 2007).
Haploview software was used for haplotype analysis, to draw the Manhattan plot and linkage disequilibrium (LD) map.
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In addition, 1,032 introduced wheat cultivars comprising 374 European, 471 American, 51 Australian, 53 CIMMYT and 83 Russian accessions were used for haplotype distribution analysis of TaGW2-6A and TaGW2-6B in global wheat cultivars (Additional file 10: Table S2).
Fluorescent fragment analysis was performed as described, taking advantage of 7 microsatellite markers used for haplotype and linkage analysis (from pter to qter: D8S255, D8S1828, D8S507, D8S260, D8S543, D8S1805, and D8S270).
We found that after ∼ 200 generations, the haplotype-based estimates were no better than the read-count-based estimates; this behavior is expected as the recombination length scale approaches the window size used for haplotype frequency estimation (200 kb in this analysis).
However, the result of this haplotype analysis should be interpreted with caution due to a limitation of the computational methods used for haplotype estimation.
SIH was then used for haplotype reconstruction.
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