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Appropriate APC-conjugated isotype controls were used for gate setting for measuring cytokine expression.
Appropriate APC-conjugated isotype controls were used for gate setting of cytokine expression.
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Isotype controls were used for gate settings.
Appropriate isotype control mAbs were used for gate settings.
Fluorescence minus one (FMO) was used for gate strategy [30].
Fluorescence-minus-one (FMO) was used for gating strategy [38].
FSC & SSC were used for gating.
For pDC counting, BDCA2+CD123+ staining was used for gating.
Fluorescence minus one control subjects were used for gating.
The unstimulated samples were used as a guide for setting the linear gates to delineate positive and negative populations for the cytokine production, whereas appropriate isotype-matched controls were used for setting the gates to quantify the percentage of T-cells expressing a specific transcription factor.
Cells were gated based on forward scatter (FSC-H) and side scatter (SSC-H) using a gate set based on experiments with DRAQ5 dye (Thermo Scientific).
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