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Four IFP fusion proteins expressed in E. coli were used for functionality analysis, resulting in successful purification by 6xHis affinity chromatography and time-dependent TEV protease cleavage of the 6xHis and IFP reporter proteins.
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A shorter version of the SF-36, the SF-12 form, is also used for functionality.
The artificial data set as well as the software used for the analysis and plotting the results that extends Matlab (R2009b) functionality is given in the supporting material S1.
Log2 titers were used for statistical analysis.
IMARIS software was used for image analysis.
These trials were used for statistical analysis.
SPECTROTYPER software was used for data analysis.
R v2.2.0 [39] was used for statistical analysis.
Mean values were used for statistical analysis.
FIRE was used for motif analysis [38].
Inferential methodology were used for data analysis.
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