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Several heterologous expression systems have been used for functional screening of just confirmation of P450 activities, including Escherichia coli, yeasts, insect or mammalian cell cultures, and plants.
The inability to identify some cellulases in function-based screening was anticipated and may be explained by a potential lack of expression of the enzymes from their native promoters in the E. coli expression host, an inability to be translated due to differing codon usage, or an inability to be secreted and/or active under the conditions used for functional screening.
To identify and isolate J. curcas genes involved in salt tolerance, yeast cells were used for functional screening.
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A similar hierarchichal approach was used for functional GPCR agonist and antagonist screens.
We developed techniques for obtaining proliferating epithelial cell cultures from bronchoscopy brushings and we used those cultures for functional screening.
Therefore, sequence homology can be used as a primary criterion for functional screening.
We selected 131 genes as potential TEC markers for functional screening using siRNA-mediated gene silencing (Fig. 2).
These nine SNPs were selected for functional screening (Table 1).
A computational approach was used for screening functional monomers and polymerization solvent in the rational design of MIPs.
A computational approach was used for screening functional monomers and polymerization solvent in the rational design of molecularly imprinted polymers (MIPs).
In this study we generated a draft complete genome sequence of Pta 11528 and used a functional screen for HrpL-dependent genes to infer its repertoire of T3SS effectors and associated Hrp Outer Proteins (Hops), which differs significantly from that of its closest relative whose complete genome has previously been published (Pph 1448A).
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