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From Biomart, a human UniProt accession number was obtained if possible to be used for functional characterization since the stickleback genes are not sufficiently annotated with regard to function.
Immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays were performed to validate one of the interactions in planta, and virus-induced gene silencing was used for functional characterization in tomato.
Over 20 independent transgenic lines were generated and used for functional characterization.
The MapMan visualization tool was used for functional characterization of differentially expressed genes.
Ex vivo preparation used for functional characterization of cutaneous afferents in response to mechanical, heat and laser stimulation.
This is particularly evident in cases when only high-throughput experiments are used for functional characterization, leading to shallow annotation graphs.
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Unfortunately, understanding the biological function of these genes is difficult due to the lack of homologs in model organisms to use for functional characterization.
EMBL-EBIs recently launched resource (http://www.ebi.ac.uk/metagenomics) uses InterPro for functional characterization of metagenomic sequences.
PK11195 [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline [1- 2-chlorophenyl -N-methyl-N- 1-methylpropyl -3-isoquinoline
Common patterns can then be identified through subgraph mining techniques, which can then be used for the functional characterization of other protein structures [ 13- 16].
The luciferase reporter assay followed by RNA secondary structure analysis was used for the functional characterization of -26 5' UTR G>A polymorphism in BRCA2.
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