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Selected 1020 genes were used for functional cDNA chip from subtracted 3469 cDNA.
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The full-length cDNA data of 10,811-non-redundant genes cloned in the normalized sorghum full-length cDNA library was used for functional analysis.
Similarly, a cDNA library cloned into a membrane tethered transcription factor was used for functional cloning of Caspase substrates in yeast [31].
cDNA synthesized was then used for cDNA-AFLP analysis and cDNA library construction.
The purified PCR products can then be used for constructing cDNA libraries.
High-Capacity cDNA Reverse Transcription Kit was used for the cDNA synthesis step.
Each library was cloned using modified linkers; ECOR1/HindIII was used for BAL cDNA, ALA for WBC cDNA, LEU for MARC5 cDNA and THR for EL1 cDNA (S1).
Adaptor-ligated cDNA products were used for direct testis cDNA selection.
Verso cDNA Ki (Thermo Scientific) was used for cDNA synthesis.
Total RNA was used for first strand cDNA synthesis using RevertAidTM RNAse H minus cDNA synthesis kit as per manufacturer's protocol (Fermentas Life Sciences, USA).
The High Capacity cDNA Archive kit (4322171, ABI) was used for cDNA synthesis.
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