Experimental designs have been used for extraction and clean-up optimization.
Maceration method was used for extraction.
Soxhelt extraction technique was used for extraction from Arisaema flavum.
Shredded materials were used for extraction.
Different PTQL queries are used for extraction.
A fivefold higher concentration of analytes used in desorption experiments, as compared to that used in extraction optimization, allows for the assumption that desorption of analytes in the latter case was also quantitative.
Three level, three variable face-centered cubic experimental design (FCD) with response surface methodology (RSM) was used for optimization of extraction in terms of maximized yields for total phenolics (TP), flavonoids (TF), anthocyanins (MA) and proanthocyanidins (TPA) contents.
Response surface methodology based on three-level, three-variable Box Behnken design was used for optimization of extraction parameters and evaluation of their effect on antioxidant capacity and total phenolic content in shell extracts.
Sonicated chromatin was used for DNA extraction (sonication optimization experiments; data not shown) or for chromatin immunoprecipitation (ChIP) assay.
As shown in Table 1, a Box Behnken design (BBD) with three independent variables, including ultrasonic power (X1), extraction time (X2), and extraction temperature (X3), was used for the optimization.
