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Expression of β-actin was used for external standardisation.
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Ceasium iodide was used for external calibration.
Effect terms are used for external control.
Vaseline is used for external use only.
Plasma and urine tramadol, M1 and M2 concentrations were determined by high-performance liquid chromatography (HPLC) with fluorescence detection and using an external standardisation procedure as no internal standard was readily available at that time.
The European standard population (Waterhouse et al, 1976) was used for age standardisation.
The reference population used for this standardisation was the complete 20 country dataset in our study.
The European standard population, truncated to 18 74 years, was used for age standardisation.
The European standard population was used for direct standardisation of the rates (Quinn et al, 2005).
The population structure used for age standardisation was the sum of the yearly population for England and Wales from 1993 to 2003.
The INDEPTH 2013 population was used for standardisation.
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