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GAPDH (Hs99999905_m1) was used for endogenous control.
GAPDH was used for endogenous control qRT-PCR reactions.
hsa-miR-191 was used for endogenous control.
Hsa-mir-191 and RNU-44 were used for endogenous control.
Assays used for endogenous control were: snoRNA142 (ID: 001231) and snoRNA234 (ID: 001234), which were chosen out of five endogenous control assays tested in our samples.
β-Actin expression was used for endogenous control with 5′-ct ggcaccacac cttctacaatg-3′ for sense primer and 5′-ggcgtacagggatagcacagc-3′ for antisense primer.
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In addition, the usage of endogenous miR-156 instead of commonly used U6 for endogenous control during real time-PCR can obtain more reliable results (Ct value: 18 25) [ 2, 42].
Pork-specific primers for the LAMP assay were designed based on the mitochondrial D-loop regions, and eukaryotic primers based on the 18S rRNA gene were used for the endogenous control.
The actin mRNA was used for an endogenous control.
The TaqMan® Copy Number Reference Assay, Mouse Tert (part # 4458368) was used for an endogenous control.
Rnu6 (U6 small nuclear RNA TaqMan® miRNA assay) was used as endogenous control for miRNA expression analyses.
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