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Other strains used for ectopic expression were UAS-E spl m8, UAS-E spl m8ACT, UAS-E spl m7ACTand UAS-E spl m7ACT
Three independent lines were used for ectopic expression assays.
Constructs used for ectopic expression included PCDNA3 [ 22, 23], and β-galactosidase [ 22, 23].
The constructs in pcDNA3.1 (Invitrogen) encoding human Capn4 and MMP2 were used for ectopic expression of these genes.
The 5053-Gal4 driver w ; P{GawB}tey5053A/TM6B, Tb+ (#2702) was used for ectopic expression in the CVM cells (Reim et al. 2012).
Standard sequences were used for both techniques.
The Heinrich classification is widely used for pathological classification of ectopic pancreas [9].
An initial serum beta-hCG cutoff level of 2,500 IU/l for expectant management can be used for asymptomatic patients with suspected ectopic pregnancy.
The book elaborates upon analytical techniques used for biofilm characterization.
Classical technique is used for speed, especially in turns.
The backscattering technique was used for collecting the emission signal.
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