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Other strains used for ectopic expression were UAS-E spl m8, UAS-E spl m8ACT, UAS-E spl m7ACTand UAS-E spl m7ACT
Three independent lines were used for ectopic expression assays.
Constructs used for ectopic expression included PCDNA3 [ 22, 23], and β-galactosidase [ 22, 23].
The constructs in pcDNA3.1 (Invitrogen) encoding human Capn4 and MMP2 were used for ectopic expression of these genes.
The 5053-Gal4 driver w ; P{GawB}tey5053A/TM6B, Tb+ (#2702) was used for ectopic expression in the CVM cells (Reim et al. 2012).
The predominant 129/SvEv genetic background was used for all studies, as no ectopic ureteric buds were observed in Bmp4Δ/+ and Bmp4Δ/f embryos in this genetic background.
Five rats were used for this study.
NHF1-hTERT cells, the immortalized cell line derived from NHF1 cells by ectopic expression of the catalytic subunit of telomerase [ 61], were used for these studies.
Pets are often used for these studies.
Clone IIB6 was used for further studies.
RAW264.7 cells were used for cytotoxicity studies.
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