Other strains used for ectopic expression were UAS-E spl m8, UAS-E spl m8ACT, UAS-E spl m7ACTand UAS-E spl m7ACT
Three independent lines were used for ectopic expression assays.
Constructs used for ectopic expression included PCDNA3 [ 22, 23], and β-galactosidase [ 22, 23].
The constructs in pcDNA3.1 (Invitrogen) encoding human Capn4 and MMP2 were used for ectopic expression of these genes.
The 5053-Gal4 driver w ; P{GawB}tey5053A/TM6B, Tb+ (#2702) was used for ectopic expression in the CVM cells (Reim et al. 2012).
This demonstrates that, despite the limitations, there could still be a role for ectopic models in drug screening, especially when its lower cost, rapidity and ease of use are considered.
The figure also shows molecular targets of the perturbation experiments that are used for model calibration, denoted by inputs u (ectopic expression of PI4KIII β (u3) and CERT (u4)), as well as activation and inhibition of PKD (u1 and u2, respectively).
Finally, their osteoinductive properties in vivo were studied in a preliminary experiment using a mouse ectopic model.
was used for the modeling.
We suggest that ectopic models and orthotopic models can be complementary in their use; with ectopic models being used in initial target screening and orthotopic models used in the validation of potential drug targets or when more subtle organ-specific aspects need to be studied.
Numerous plastic materials are used for modeling.
