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A different primary antibody is used for each target protein.
The process begins, one annotated slide suggests, when an NSA supervisor signs off on search terms – called "selectors" – used for each target.
Number of HMM states used for each target class.
We investigated the performance of the system with changes in the following parameters of the system, Number of HMM states used for each target class.
Approximately 26 to 35 cycles of amplification were used for each target at 95°C for 30 seconds, 53 62°C for 1 min and 72°C for 1 min. The number of cycles corresponds to the linear portion of the exponential phase for each gene expression and was assessed in preliminary studies.
In an ideal case, a suitable σ a should be used for each target MHC molecule.
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These optimization and validation experiments are performed on cDNA exactly as suggested by ABI in order to find the correct concentrations of primers and probes to use for each target, as well as the optimal useful dilution of cDNA template (found to be 1 5 for our purposes) to use that would allow each PCR to proceed with optimal efficiency.
analyzing Test Plate results to identify the target-specific dilution ranges within which each different target generates a near-ideal slope (e.g. -3.3219) while exhibiting LOG-linear behavior, then, diluting all samples into these multiple ideal ranges so they can be used for each different target within each target's confirmed useful dilution range.
Ten times dilution series were used for each known target, which were amplified using SYBR-Green qPCR.
Primer sequences that were used for each gene target are listed in Additional file 3, Table S2.
First, it would be useful to limit the number of the meteorological elements used for estimating each target meteorological element.
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used for each well
used for each platform
used for each database
used for each item
used for each condition
used for each species
used for each test
used for each animal
used for each gene
used for each marker
used for each class
used for each sample
used for each measurement
used for each block
used for each case
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