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One tray was used for each replication and each treatment was replicated three times, following a completely random design.
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Two biological replicates of cotton bolls from TM-1 and im mutant were harvested at 10, 17, 24, 28, and 44 DPA before 9 A.M. The developing fibers from 10 30 ovules that were harvested from 2 4 different cotton plants used for each biological replication.
Sixty seedlings with a similar height and crown size were used for each treatment with 15 replications.
Three replications were used for each experiment, with nine individual plants per line evaluated for each replication.
The reference and target gene primer sequences are shown in Additional file 7. Three biological replications and five technical replications were used for each time-point sample.
Five neonates (younger than 24 h old) were used for each concentration based on 4 replications.
Three replications were used for each cultivar.
Three biological replications were used for each time-point sample.
Three biological replications were used for each treatment.
Three replications were used for each sample set.
Three biological replications were used for each treatment and at each time point.
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