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SAS V.9.2 was used for random slope random intercept models and STATA V.11 for all other analyses.
The seeds used for each simulation were true random numbers provided by random.org via the package "random" [randomNumbers: [ 31].
Ten-fold cross validation was used for each optimisation trial, with a random subset of 50% of the data being used to fit each new tree.
The tissue samples from all of the groups were coded and studied independently by 2 observers (C.P.V and C.C). in a blinded fashion; sections selected at random were used for each animal, and 3 4 fields per section were studied.
Different seeds were used for the random number generator at each replication while keeping it the same across different methods.
Also, the Matlab© software is used for generating random draws.
Excel was used for the random selection of the groups.
One hundred and forty random primers were used for random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR).
To initiate reverse transcription, random hexamers were used for random priming and a oligodT primer 5' GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3' for polyA-specific transcript priming.
RNA from aggregates of each condition (2 μg each group) was used for random hexamer primed cDNA synthesis using BioScript reverse transcriptase (Bioline GmbH, Luckenwalde, Germany).
Clontech Diversity PCR Random Mutagenesis kit was used for random mutagenesis, in conditions optimal for 7 mutations per 1000 bp.
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