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The sets of three wells for the MTT assay were used for each experimental variable.
In-spite of this at least 26 explants were used for each experimental condition, much higher than previous studies (Chu et al. 2008; Chu et al. 2006).
Computing sample size from statistical theory can be a major challenge because the theoretical distributions of values are complex and asymmetrical, and a different formula needs to be used for each experimental design.
The effect of TOS experiments on the C7 conversion, hydroisomerisation, hydrocracking, and cyclic product yields at different reaction temperatures from 210 to 250 °C is shown in Fig. 16a d, respectively, with fresh catalyst used for each experimental run at the different reaction temperatures.
Three biological replicates were used for each experimental condition.
At least 3 wells were used for each experimental point.
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Eight animals were used for each comparative experimental condition.
The new syringes and filters were used for each sample and experimental point.
The volume ratio of algae biomass to fermenting bacteria (AB/FB) ranged from 0 (fermenting bacteria alone) to 1.0, and 50 mL fermenting bacteria (dry biomass = 3.4 g L-1) was used for each of the experimental variations.
For each of these, hearts were randomly distributed into six experimental groups for perfusions: baseline control (11 mM glucose) ± either 5 μM lactacystin or 10 μM MG-132; and high glucose (33 mM glucose) ± either 5 μM lactacystin or 10 μM MG-132 (n = 8 rats were used for each of the experimental groups indicated).
On the second visit, one-repetition maximum (1RM) for four exercises was measured to determine the weights to be used for each exercise on the experimental days.
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