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Separate models were used for each developmental domain.
A minimum of three biologically independent samples were used for each developmental stage, and two technical replicates were performed on every sample.
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Total RNA was isolated from An. stephensi of the same age or developmental stage and used for each blot.
The chicken chorioallantoic membrane (CAM) assay has been widely used for developmental and post-developmental studies of angiogenesis because of the easy access to the vascularized CAM [ 28, 29].
Different animal models are used for assessing developmental effects of MH in offspring.
Using two cases of on-farm biogas production; one from Finland and one from Brazil, we demonstrate how such analysis can be carried out and used for assessing developmental possibilities.
The endpoints used for assessing developmental toxicity were recorded and described for embryos in both the control and treated groups [30].
This guide can be used for describing developmental series of any vertebrate species or for documenting specimen variability of a particular species.
Different methods have been used for detecting developmental defects of enamel (DDE).
The CAM assay is widely used for both developmental and post-developmental studies of angiogenesis due to easy access to the vascularized CAM membrane [ 24, 30, 39].
For instance, 'Omics' technologies, including transcriptomics, proteomics, and metabolomics have begun providing evidence that viable oocytes/embryos possess unique molecular profiles with potential biomarkers that can be used for the developmental and/or viability selection [ 2].
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