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Primary human monocytes were extracted from the blood samples of anonymous healthy male donors, donated by the blood transfer centre of the Luxembourgish Red Cross and were used for diverse experiments in agreement with the convention between the Luxembourgish Red Cross and the University of Luxembourg from 16.05.2011 and following the principles of Helsinki Declaration.
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The RNA obtained using this method has been used for diverse downstream experiments including RNA-Seq, RT-PCR, and qPCR.
First of all, he said, a single software program could be used for diverse efforts.
Such repetitive sequences can be used for diverse biotechnological applications.
Because the diverse disease models used for these microarray experiments (including diabetes, cancer cachexia, chronic renal failure, fasting and denervation) have muscle atrophy in common, the commonly up- or downregulated genes are believed to regulate the loss of muscle components and are called atrophy-related genes or 'atrogenes' (Sacheck et al., 2007).
Male mice were used for all experiments.
These cells were used for subsequent experiments.
The supernatant, containing soluble proteins, was used for trapping experiments.
Single-cell clones were expanded and used for further experiments.
(b) Floor plans of the rooms used for our experiments.
Different groups of mice were used for different experiments.
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