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Subsequently, chemiluminescent substrate (ECL) (Thermo Scientific) was used for detection.
HRP-conjugated secondary antibodies were used for detection.
Immunostaining was used for detection of ciliary axonemes (Arl13b) and for subcellular localisation of CEP290.
A goat anti-mouse secondary antibody (Vector Laboratories Inc., Burlingame, CA) was used for detection.
OptiView DAB (Ventana Medical Systems, Tucson, Arizona) was used for detection.
(c) RT-PCR strategy with primers used for detection of ATXN8OS CUG transcripts.
Specific HRP-conjugated secondary antibodies followed by enhanced chemiluminescence (Amersham Pharmacia Biotech) were used for detection.
The Refine polymer detection kit (Leica Biosystems) was used for detection.
Gomes, A., Fernandes, E. & Lima, J. L. Fluorescence probes used for detection of reactive oxygen species.
These structures can be used for detection purposes.
The cells' medium was half changed every other day and was used for detection after being cultured for 1 week.
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