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ChromasPro (v.1.33) and sCAP3 software packages were used for consensus alignment for the nucleotide sequences.
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These sequences were used for multiple alignment (ClustalW with default settings) and consensus sequence calculation.
The RALEE mode [ 41] in the emacs editor was used for visually inspecting alignments, consensus structures and conservation patterns.
The forward and reverse sequence reads for each genotype for a particular primer pair were assembled using Contig Express (Invitrogen, USA) and the consensus sequence was used for alignment.
SeqManPro (Lasergene 8.0, DNASTAR) was also used for sequence alignments containing BAC assembly consensus sequences and predicted gene sequences.
These consensus structures along with their primary sequence information were then used for alignment with the query sequence.
Please see the legend key provided at the end of the alignment for meaning attached to color-code resides and letters used for writing consensus sequence.
CLustalW was used for nucleotide sequence alignment.
MAFFT was used for alignment.
The Prosite database PDOC00028 alignment was used for the reference consensus sequence of general C2H2.
Our "ancestral" sequences are similar to consensus sequences used for representing protein multiple alignments.
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