Electrical conductivity (EC) of 293 surface soil samples taken from 2 km × 2 km grids in 2007 and 40 soil profiles acquired in 2008 was analyzed and used for comparative mapping.
Our results with A. convolvuli clearly show that M. sexta BAC probes can be used for comparative mapping in non-congeneric species.
However, it can also be used for comparative mapping of genomic fragments onto the chromosomes of closely related species, without additional cloning or DNA sequencing, as shown in [27], [28].
Selected homologs from the Pinaceae and C. japonica linkage maps were used for comparative mapping.
Therefore, these SNPs can be used for comparative mapping between the Clementine and sweet orange.
The map could also be used for comparative mapping with other genera of Pinaceae.
However, the transferability of these SNPs to wild forms of V. vinifera was lower (18%), which will impact their use for association studies or selective sweep identification, and the transferability was even lower (2.3%) in non- vinifera Vitis species, which will affect their use for comparative mapping (Table 1).
In order to demonstrate the usefulness of anchored maps to compare QTL locations across mapping populations, the two major QTL for crown rust resistance on Lolium LG 1 detected in TC1*SB2 [ 25] and Xtg-ART [ 23] were used for comparative QTL mapping.
Using bioinformatic approaches, a large set of single-copy orthologous genes (COSII) shared between Asterid plant species (including C. canephora and S. lycopersicum) and the model Rosid plant genome A. thaliana has been identified [ 7] and used for comparative genetic mapping and chromosomal evolution studies in the Solanaceae [ 10- 13].
Five Solanum species, including S. verrucosum (A genome, PI 275260), S. cardiophyllum (B genome, PI 347759), S. chromatophilum (P genome, PI 365339), tomato (S. lycopersicum, cv MicroTom) (T genome), and S. etuberosum (E genome, PI 558288), were used for comparative FISH mapping of the telomeric and subtelomeric repeats.
Due to the sharp increase of EST sequences deposited in NCBI, more and more EST-SSRs have been identified and used extensively for comparative mapping, DNA fingerprinting, biodiversity, and evolutionary studies in lots of species [ 19, 20].
