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The original method [5] was also used for comparative experiment results.
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A published construct with FMC-63 mAb-derived CD19-specificity was used for comparative experiments (Rossig et al, 2006).
The dengue 3' SL has significant secondary structure [4], [5], therefore the structured alfalfa mosaic virus (AMV) 3' untranslated region (UTR) RNA [20] was used for comparative competition experiments.
This version of the protein was used for comparative binding experiments.
We evaluated these in the same total RNAs used for comparative microarray experiments.
For RT-PCR, 7 μg of the total RNA pools used for comparative microarray experiments were reverse-transcribed using M-MLV transcriptase (Gibco BRL, Gaithersburg, MD, USA) and random hexamer primers (Amersham).
Model fish species are also increasingly used for comparative studies in experiments with other fish species that are of importance for aquaculture, e.g. as a model for the effects of swimming exercise on muscle development [ 67].
Affymetrix® gene expression data used for comparative analyses in this study was obtained from previously published experiments [ 10].
Most of the top 20 most-abundant genera in each experiment (Supplementary Fig. S4) were also categorized as those in the two other experiments, and a total of the top 26 most-abundant genera were used for comparative analysis.
Ionization chambers were used for comparative measurements.
In the experiments, the classical polar code composed by Arikan (i.e., l = 2), which is denoted by polarcode 2, is also used for comparative analysis.
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