Exact(3)
An increase in the size of mRNA could be due to alteration in mRNA cleavage (the DSE mutation results in a CA sequence doublet that is frequently used for cleavage in the context of a poly(A) site), alteration in polyadenylation (fraction of mRNA polyadenylated or the length of ploy(A) tail), or increase in unprocessed (extended) transcripts.
Cyclic SBL is thus identical to standard SBL except that there is a deoxyinosine incorporated in the query primers that is used for cleavage.
We found that incorporation of a carbonylated amino acid at the N-terminus does not allow removing the acetal group even after 24 h of incubation, in the mixture used for cleavage or microwave-assisted TFA (Kluczyk et al. 2010) cleavage from the resin.
Similar(5)
Cerium (IV) trihydroxyhydroperoxide (CTH) as a packed oxidant reactor has been used for oxidative cleavage of FA into highly fluorescent product, 2-amino-4-hydroxypteridine-6-carboxylic acid.
Supernatant fractions of ultracentrifuged cell extracts were used for TEV cleavage experiments and protein purification.
The RNA transcripts used for the cleavage and binding assays were generated by T7 RNA polymerase using oligonucleotides as templates.
After incubation, samples were either used for substrate cleavage assays for caspase-9 (LEHDase) and caspase-3 (DEVDase) or for caspase processing by Western blotting.
This plasmid was then converted to an integrating plasmid by ligating the pRS306 [29] large AlwN1 fragment to the pKG16 large AlwN1 fragment, resulting in the pKG17 plasmid used for Spc110 cleavage strain construction.
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