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Currently, every imaging technique used for cell tracking has merits and defects.
SimiBiocell software was used for cell tracking (Bischoff et al., 2008).
Bioluminescence imaging is commonly used for cell tracking in SC transplantation studies [ 78, 79, 91] (Figs. 3 and 5).
At present, MRI, scintigraphic tracking, quantum dots (QDs), reporter gene labeling and optimal imaging, have been widely used for cell tracking.
It is currently not known if SPION can be used for cell tracking during the progression of experimental arthritis nor the evaluation of RA treatments effects in vivo.
GFP labeled rAT-MSCs, used for cell tracking after injection, were double-stained on sections for GFP, and antigen of interest.
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The PKH is an extensively used supply for cell tracking protocols [ 26, 27, 34], which could be used associated with other methodologies such as flow cytometry and two-photon excitation microscopy.
Therefore, we strongly suggest that FMNPs can be safely used for cell labeling and in vivo tracking and imaging within 60 μg, and do not affect cell function and mice lifespan.
Chen et al. reported a nanoprobe in which NIR979 conjugated onto the surface of PLL-capped SPIO-PAA nanoparticle was used for dendritic cell tracking [12].
Accordingly, SPIO particles have successfully been used for clinical cell tracking in melanoma patients [ 80].
Expression of the HSV1-tk reporter gene can be used for living cell tracking by positron emission tomography (PET) or single-photon emission computed tomography (SPECT), depending on the types of radionuclide-labeled substrates.
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