Last, the read counts of the alleles in the same protein group are summed non-redundantly and used for candidate allele selection (step IV in Figure 1).
The results of the present study have substantially extended comparative knowledge of the field pea and M. truncatula genomes, and such information may be used for candidate gene selection for further application to breeding programs.
The following bold terms were used for candidate gene selection: response to abiotic stimulus(GO 0009628); response to biotic stimulus(GO 0009607); response to endogenous stimulus(GO 0009719); response to extracellular stimulus(GO 0009991); response to stress(GO 0006950).
The criteria to be used for candidate evaluation and selection should be described.
CRISPR-mediated mutagenesis might be an approach to independently confirm knockdown phenotypes and it could be used as a general strategy for candidate selection of RNAi screens in future.
This HMM is used in a HMMER search of the input protein set [HMMER 2.3.2 (Eddy, 1998)] using a relaxed cutoff (HMM E-value for candidate selection, Table 1; Fig. 1.1), resulting in a reduction of the search space for subsequent steps by ∼95% without loss of divergent kinases.
cAF marginally outside target range for candidate selection.
As gene expression changes may be used for selection of candidate disease causing genes [ 9, 61], we evaluated group specific and individual gene expression profiles.
In addition, the Milan criteria (single tumor ≤5 cm in size or ≤3 tumors, each ≤3 cm in size and no macrovascular invasion) have been most widely used for the selection of candidates for liver transplantation for more than a decade.
We will also introduce new standards to encourage work on, and publication of, disease models and mechanisms that can be reliably used for the selection of drug candidates to be entered into the expensive and time-consuming next phase of drug development.
