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CNVPartition v 3.1.6 is the CNV-calling algorithm used for calling the aberrations in the samples.
SAMtools takes into account mapping qualities, base qualities and error rates (from raw sequence quality scores) in approximating the posterior probabilities of consensus genotypes, which are then used for calling the variants [ 17].
To obtain estimates for these prior weight parameters, we first note that their estimation only requires estimates for the two thresholds used for calling the three states, since the weights for a given sample are determined given a choice of thresholds.
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Nevertheless, regardless of the thresholds used for calling MIs, the mean number of heterozygous SNPs within MIs was found to decrease significantly over time (Supplementary information, Fig. S1).
Design scores could be generated for only 34% (95,478/278,979) of the target SNPs submitted to Illumina, primarily because of the permissive probability threshold (PF = 0.10) used for calling variants in the flanking sequences.
Variant calling was performed using the Probabilistic Variant Detection tool and adjusting the options specified in CLC to the parameters used for calling high quality variants of V. cholerae [ 18].
This discrepancy may also be due to differences in the thresholds used for calling MIs.
We used the following commands for calling the variants using SAMtools.
A poll published a day before the decision found that forty-eight per cent of Americans opposed letting cell phones be used for calls in the air, while only nineteen per cent supported it and thirty per cent were neutral.
This is important since the set of individuals used for variant calling influences the called genotypes and therefore a bias might be introduced in this study.
PennCNV software was used for the calling of CNVs, which uses the combined values of log R ratio and B allele frequency values and an integrated hidden Markov model [27].
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