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Here the IntelliCage, an automated system, was used for behavioral testing of APP female transgenic mice with both the Arctic and Swedish mutations, the tg-ArcSwe model.
Three owls were used for behavioral testing.
The animals used for behavioral testing above were sacrificed at either 6 weeks or disease end point, whichever came first.
After the completion of testing, rats used for behavioral testing were deeply anesthetized with sodium pentobarbital and their brains were removed and fixed in 10% paraformaldehyde overnight and then cryoprotected in 20% sucrose for at least 2 d.
Both males and females were utilized for the histological assays, while only males were used for behavioral testing.
D/+ heterozygotes (n = 9) and wild-type littermates (n = 9) were used for behavioral testing beginning at 8 weeks of age.
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Another 12 mice were used for behavioral tests.
Mice used for behavioral tests were backcrossed to the C57BL/6J background for more than 6 generations as described in Methods section and diagramed in supplementary Figure S4.
Animals used for infarct analysis on day 28 (8 animals each) were also used for behavioral tests.
A 200 × 80 × 80 cm wind tunnel was used for behavioral tests.
Six flies from each group were used for behavioral tests and the remaining fly was subjected to hydrocarbon analysis by Direct Analysis in Real Time mass spectrometry to monitor effective transfer of the test compound to the flies.
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