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This element was also expressed in the tritocerbral PDF cells of late pupae and newly emerged adults, however these neurons undergo apoptosis shortly after adult emergence and were therefore not present in the adult flies used for behavioral experiments ([28]; P.H. Taghert, unpublished data).
Mice used for behavioral experiments were from the N3 generation.
Eight to ten-week-old male C57BL/6J mice were used for behavioral experiments.
Zebrafish used for behavioral experiments were maintained in individual 2 L aquaria.
These emerged adults were used for behavioral experiments before they had any contact to flowers.
All flies used for behavioral experiments were outcrossed into our isogenic background (w 1118 ) for at least five generations.
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Five had been used for behavioral and neurophysiological experiments, eight had been experimentally exposed to ethanol (Wright et al., 2013), and two had been terminally anesthetized for several days.
Some rats used for behavioral data were not included in the biochemical or histological assays below, but were used for other experiments not included in this study.
ClockLab software was used for behavioral analysis.
Three owls were used for behavioral testing.
A total of 44 animals obtained in five separate batches were used for independent behavioral experiments.
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