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D1RKD and age-matched WT male mice (7 28 weeks old) were used for behavioral analyses.
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ClockLab software was used for behavioral analysis.
Three owls were used for behavioral testing.
Seven animals in each group were used for behavioral analysis.
Another 12 mice were used for behavioral tests.
Mice used for behavioral experiments were from the N3 generation.
Brain slices were prepared for one of 2 analyses: 1) densities and diameters were measured for local Fos plumes caused by microinjections of CRF or vehicle in a group of rats not used for behavioral tests; 2) microinjection centers were mapped by ink microinjection and cresyl violet staining in all rats used for behavioral testing.
The statistical analysis employed ANOVA for the body weight variation, body fat weight, and biochemical analyses, while the Kruskal-Wallis test was used for the behavioral analyses, with a 5%% level of significance.
Male mutant and control littermates were used for all behavioral analyses.
Male mice from the Mouse Diversity Panel (HMDP) were used for all behavioral analyses.
Male mice of the C57BL/6J genetic background were used for all behavioral analyses.
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