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Clone sequencing was used for array validation.
Pairs of primers that were used for array validation are shown in Table 1.
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The validation set comprised not only RNA of the biopsies used for array analyses but also an additional set of 8 CIN3 and 9 CxCa.
was used for array analysis.
Among the samples used for validation of this array was meningioma T24 DNA, which is a well characterized tumor containing a large terminal deletion of 22q and an additional approximately 150 kb biallelic deletion involving EWSR1, GAR22, RRP22, AP1B1, RFPL1 and NEFH genes [ 37, 38].
Genomic qPCR, dye-swap array-CGH analysis and B-allele profile analysis were used for their validation.
The tumor samples used for SNP array genotyping and mutational rate validation of candidate genes are shown in Figure S2.
Additionally, two in- dependent AML gene array cohorts were used for validation.
The 139 prototypical arrays for PAM50 development were used as training dataset, and three independent microarray studies with Han Chinese origin were used for independent validation (n = 535).
Genes used for microarray validation.
The training set was used for internal validation and the validation set for external validation.
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