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Vitek2 was used for antimicrobial testing.
Epsilometer-test (Etest®) (bioMerieux, Durham, NC, USA) was used for antimicrobial testing of doxycycline, tetracycline and minocycline.
Microorganisms used for antimicrobial testing were obtained from the culture collection center, Thailand Institute of Scientific and Technological Research (TISTR), Thailand, as follows.
Isolates were subcultured onto GC agar base (Difco, Detroit, MI) supplemented with 1% IsovitaleX™(Oxoid, USA) and pure cultures swabbed, suspended in tryptone-based soy broth and frozen (−70°C) until used for antimicrobial testing.
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Broth micro-dilution method was used for antimicrobial test.
To study efflux, we loaded cells with berberine or the combination of berberine with the compound at concentrations significantly higher than those used for antimicrobial susceptibility testing and then transferred them into fresh medium.
The Kibry Bauer disk diffusion method was used for antimicrobial sensitivity testing.
Each laboratory reported annually, which methods were used for antimicrobial susceptibility testing.
Each participating laboratory reported annually which methods were used for antimicrobial susceptibility testing.
The medium BBL™ CDC Anaerobe 5 % Sheep Blood Agar (Becton Dickinson, Heidelberg, Germany) was used for antimicrobial susceptibility testing for the general growth of anaerobes.
The medium used for antimicrobial susceptibility testing of RGM was BBL cation-adjusted Mueller-Hinton (CAMH) Broth (BD, Franklin Lakes, NJ, USA).
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