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Pools of 3 different siRNAs were used (final concentration 25 nM) for triplicate wells for each siRNA pool.
For Fiber-FISH experiments, two-fold the labeled DNA probe and Cot1 competitor DNA were used (final concentration of 80 ng/μL of each probe).
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Different types of organic load were used (final concentrations): ▪ No organic load (aqua bidest).
Survival experiments were then performed as previously described using final concentrations of 1 and 30 mM H2O2.
Hygromicin B (Invitrogen; 50 mg ml 1 stock solution) was used at final concentration of 200 µg ml 1.
The drug brefeldin A (Sigma) was dissolved in ethanol and used at final concentration of 2 μg mL−1.
Doxycycline (Sigma-Aldrich) was dissolved in water (10 mg ml 1 stock solution) and used at final concentration of 2 µg ml 1.
Latrunculin A (Sigma) was used a final concentration of 0.2 mM.
All primary antibodies were used at final concentration 1 μg ml−1.
For salt stress, sodium chloride was used at final concentration of 200 mM for 3 hours.
Antifoam was used at final concentration of 0.5 mL L−1 (Dow Corning Antifoam RD emulsion, VWR International Ltd., Poole, UK).
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