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A two-step process was used; extraction of alkannin derivatives with supercritical CO2 followed by alkaline hydrolysis of alkannin derivatives.
As is the case with abiotic material, not only the used extraction of biotic material is considered but also all organic material that is taken from nature (including biotic material taken from agricultural systems).
In order to build the database, a variety of methods for importing records en masse will be used: extraction of records from library catalogs, such as the AMNH's; extraction from literature indexes such as PubMed; and optical character recognition software together with custom text-processing scripts, for importing the bibliographies of print works.
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Another study by Mavragani [[40]] compared straight wire to standard edgewise using extraction of premolars.
Those mRNA species whose abundance changed concordantly among the patients during the 7-day window surrounding the date of diagnosis were identified using extraction of Differential Gene Expression (EDGE) software [23].
The most used extraction solvent of polyphenol from vegetable matrix is methanol or ethanol and water-methanol/ethanol solutions [37].
By this method, stilbenes in grape canes was extracted 10 min only using extraction temperature of 75 °C and ethanol (60%) as the extraction solvent.
About 20 mg lyophilized mycelia were ground in liquid nitrogen and used for extraction of DNA using the genomic DNA extraction kit (GenMark Technology Co., Taichung, Taiwan).
This lyophilized dry biomass was weighed and used for extraction of total lipid using bead beater.
PubMed database was used for extraction of relevant publications using MeSH terms.
The cell pellet was used for extraction of total genomic DNA using the DNeasy Blood and Tissue Kit (Qiagen, Germany).
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