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When stated that BacT/Alert was used, experiments were performed at the division for Clinical Microbiology in Kalmar.
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Mice of both sexes, aged 6 12 weeks were used, and experiments were performed on age- and gender- matched groups.
Molecular weight analyses using SEC experiments were performed with a HiLoad 16/600 Superdex 75 pg column.
To confirm our results using human cells, experiments were performed using HEK 293T cells and adult human dermal fibroblasts.
Adult male Sprague Dawley rats were used and all experiments were performed in accordance with the UK Animals Scientificc Procedures) Act 1986.
Immunoblotting experiments were performed using established methods.
SPR experiments were performed using a Biacore X biosensor (GE Healthcare).
Experiments were performed using a Ag/AgCl reference (ΔEref=±1 mV).
HS-AFM experiments were performed using a laboratory-built instrument57.
Experiments were performed using cells from five cattle.
Experiments were performed using three biological replicates of culture and transfection conditions.
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