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We used divergence in ancestral repeats as a neutral reference to estimate the constraint on 4-fold degenerate sites of avian genes in a whole-genome approach.
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We use divergence in throat color morph frequencies and compare that to neutral genetic variation to infer the evolutionary processes acting on islet- and mainland populations.
The main conclusion from this study is that 4-fold degenerate sites of avian genes evolve under significant constraint, at least when constraint is estimated using divergence in ARs as a neutral reference.
Accordingly, several methods use divergence data in combination with SFS data to estimate the fraction of nonsynonymous divergence that is driven by beneficial mutations.
We also inferred the DFE using the method described by Eyre-Walker and Keightley (2009), which uses divergence data in addition to segregating sites.
Thus, much of the adaptation detected using divergence data might have in fact occurred in an ANC whose population history differs substantially from that of the current population.
The maximum-likelihood test used to detect positive selection using divergence data is powerful only in situations in which the gene has experienced recurrent selection events at the coding sequence; adaptation at regulatory sites, however, cannot be detected using this method.
Our proposed clustering method uses divergence measure to discover clusters in the network.
Even though our yeast strains can be regarded as different "species" because they do not mate with each other, they can also be considered the same species if one uses the divergence in ribosomal RNA sequences to define species (a field standard)!
To avoid the saturation effect as much as possible, we used the divergences in Group B with dnuc = 1.9±0.9% and dmt = 3.3±0.1% (Figure 2).
We inferred phylogenies using parsimony-based and probabilistic approaches to evaluate uncertainty in topology, test deviations from taxonomic classifications, and generate a distribution of phylograms to be used in divergence time estimation (see below).
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