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The used concentration was 100 times higher than the microbial burden found in hospitals [ 2].
Free KDO in the used concentration was inactive in regulation of TLR4, CD11b and CD14 expression and did not induce TNFα release but its impact in biological activity was detected when KDO was applied as constituent of Re-LPS.
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The used concentration is approximately 25 times higher for cement and 20 times higher for bone debris compared to findings in TKA during surgery [ 21].
The observed diversity of the used concentrations was considerable.
The formed stock solution has a known concentration from which all used concentrations are taken via dilution.
To ensure the inhibition of the enzyme, 10 eq. of the inhibitor versus the POP enzyme were used (concentrations were previously tested in other systems).
All the used concentrations are determinate in previous experiments of toxicity.
The list of tested inhibitors, including the used concentrations, is shown in Table 1.
For the antigen-blocking tests, the used concentrations were: intact bovine aggrecan at 333 μg/ml and ARGSVI-peptide at 200 μg/ml.
Although they have been detected in groundwater, under multiple land uses, concentrations were at levels below documented safe drinking standards.
"If an animal is trying to use concentration, it might be very misleading," she said.
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CEO of Professional Science Editing for Scientists @ prosciediting.com