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For some pull-down assays Unc45bFlag was not eluted and used bound to the agarose beads.
The primers used bound to exon 3, in particular -18 and +26 bp upstream and downstream of start and stop codon, respectively (forward primer: 5'-TGCCTGGGAAGTTTGAGCTG-3'; reverse primer: 5'-CAGGTTGCCAGGCAGGATG-3').
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This allowed us to define the expression profile of the complete NBPF gene family in neuroblastoma cell lines, as the primers we used bind to regions that show high sequence identity between the different NBPF paralogs.
The Prn monoclonal antibody (MAb) (PeM85) that was used binds to the linear epitope GGFGPGGFGP present in the repeat region 1 of all known Prn variants, except Prn13 (15 ).
Indeed, the pan-cytokeratin antibodies (AE1/AE3) we used bind to multiple cytokeratins present on both human epithelial and mesenchymal cells [ 34, 35].
We first obtained a lower bound on the achievable rate and then used this bound to optimally allocate power between the training and data transmission phases.
VecTest uses antibodies bound to gold sol particle labels, while the RAMP test uses antibodies bound to fluorescently labeled latex particles.
The targeting antibody used binds preferentially to pRBCs containing late maturation stages of the parasite.
Cyclin E1/CDK2 kinase activity was determined using immunoprecipitated proteins bound to antibody-Sepharose beads [32].
On the other hand, I felt strictly bound to use exact details about the Koenigsberg airstrip.
Energy exports are Russia's new strength, but it does not follow that Russia is bound to use them irresponsibly.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com