The deionized (DI) water used was prepared in our laboratory.
The software used was prepared by the researchers.
AAO templates used were prepared by aluminum anodic oxidation as described previously [17].
All bacterial suspensions used were prepared in DMEM.
The merozoite surface protein antigens used were prepared as recombinant proteins in Escherichia coli.
All the washing solutions used were prepared in MilliQ water (Millipore, MA, USA).
The inocula used were prepared from overnight growth cultures in BHI-broth.
The dark glass bottles used were prepared at Skanderborg Pharmacy, Denmark, and all contained 20 doses of 0.5 ml of vegetable oil.
All media used were prepared as for S. cerevisiae: glucose and glycerol-based complete media, broth and solid.
Except for the commercially available superoxide dismutase (SOD, from bovine erythrocytes, Sigma), all protein samples used were prepared using human origin constructs.
Land use was prepared with the Google earth images for the year 2013.
