Sentence examples for used at the same dilution from inspiring English sources
For co-localization assay, the rabbit anti-MPS1 serum raised against bacterial expressed recombinant PfMSP1 protein and the goat secondary antibody coupled to Alexa 500 nm were used at the same dilution.
The secondary antibody, a biotinylated goat anti-mouse immunoglobulin serum, was used at the same dilution.
Antibodies were used at the same dilution as for the germ line staining.
The negative control MAb #4 F11 directed against parainfluenza type 3 N protein [ 41] was used at the same dilution.
Furthermore, mouse anti-chicken α-actin (Amersham, Madrid, Spain) was also used at the same dilution to examine the relative expression of the other proteins.
Anti-GFP and anti-GRP78 were used at the same dilutions as the control.
Control sera from pSM214-treated or untreated rabbits were used at the same dilutions as IL-1Ra-containing serum.
Secondary antibody IgG FITC from Santa Cruz Biotechnologies was used in the same dilution 1 50 with an incubation period of 45 min at room temperature.
On the other hand, no antisera exhibited 50% neutralization in a TZM-bl assay using HIV-1 isolate Bal or a p24 assay using isolate IIIB at the same dilution.
Thereafter, blots were blocked by immersion at room temperature for 1 h in ProtoBlock solution (National Diagnostics Inc., UK) and probed with the identical antibody used for immunohistochemistry and at the same dilution.
Bands recognized by the anti-rTsTrx-1 serum were visualized with 3,3′-diaminobenzidine (DAB) and H2O2 as substrate; normal rabbit serum was used as negative control at the same dilution.
