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Inspectors also found that chemical weapons were "probably used" at a further four sites in Syria.
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Primary signals were then detected with either alkaline phosphatase-conjugated anti-rabbit secondary antibodies (for Pfk1) or alkaline phosphatase-conjugated anti-mouse secondary antibodies (for Pgk1), both used at a 1 20,000 dilution, and further revealed with chromogenic substrate solution BCIP/NBT (Sigma).
For each child, the mean weekly participation units in organised sports activity during the study period was calculated, and further used at a group level for analyses.
Dynasore, a dynamin inhibitor, was used at 37°C to further explore active transport across the cells.
Bacteria can easily be revived and used in a further study at later time points.
However, to be used clinically, at a minimum, further analyses to determine cut points for the diagnosis of retinopathy would need to include sensitivity and specificity analyses.
Stock solutions were further diluted and used at 50 μM.
Following these screening assays, LACE extract was used in further experiments at a concentration of 250 μg/ml.
Viable confluent cell cultures were used for further passages at a ratio of 1 1 to 24-well microtiter plates (BD Falcon).
Overall, 6.0% of children aged 11-17 in England had used a sunbed at least once; a further 14.9% said they might do so in the future (table 3).
SMCs were detached from the tissue culture plastic using trypsin/EDTA and further plated at a density of 10 cells/cm in tissue culture flasks to allow for expansion.
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