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A preparation of the B. subtilis ribosomes34 was used at a final concentration of 1 μM.
BrdU was used at a final concentration of 20 μmol/mL.
All Mn-oxides were used at a final concentration of 1.82 mM.
Blebbistatin was used at a final concentration of 25 µM.
Primers were used at a final concentration of 1 µM.
Cycloheximide (CHX, Sigma) was used at a final concentration of 50 µg/ml; phosphonacetic acid (PAA, Sigma) was used at a final concentration of 500 µg/ml.
PAA, an inhibitor of DNA synthesis, was used at a final concentration of 250 µg/ml.
MG132 (VWR) was used at a final concentration of 12.5 µM.
siRNA's were typically used at a final concentration of 100 nM.
Caspase inhibitors were used at a final concentration of 25 µM in DMSO.
All antigens were used at a final concentration of 1 µg/ml.
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