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At day 6 of culture, nonadherent cells and loosely adherent DC aggregates were harvested, washed, resuspended in RPMI 1640 supplemented with 5% FBS and used at a density of 2×105 cells/ml for experiments unless mentioned otherwise.
Dictyostelium cells growing in suspension were harvested, washed, resuspended in Soerensen phosphate buffer and used at a density of 1×105 cells/ml for inoculation of the E. coli B/r suspension.
Cells were harvested with cold PBS (Invitrogen), washed, resuspended in DMEM supplemented with 10% FBS and used at a density of 2×105 cells/ml for experiments unless mentioned otherwise.
During each assay (see below, Section 2.2) cells were used at a density of 1000 μL−1.
For experiments, cells were used at a density of 106 cells/ml and kept in medium without rHu-EPO for 1 hour before the start of the experiment.
Contour mode 1 is a threshold based algorithm used at a density of 0 mg/cm so all voxels could be included within the ROI to describe total bone.
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This is because the hydrogen and oxygen flow rates are completely used at a current density of 1.2 A/cm2 for this case.
When the Lallemand LYCC 6469, Tembec T1, and T2 yeast strains were used at a high cell density of at least 150 × 106 cells/ml both the furfural and HMF present in the glucose supplemented, hemicellulose rich water soluble fraction were quickly removed.
In practical ecological engineering, we suggest that silver carp can be used at low density (20 g/m3) as a biomanipulation tool, despite their potential to deteriorate water quality.
Perhaps one molecule could be used at low density to immobilize the filaments, and a second could be added in solution?
Percoll and Ficoll have usually been used at densities of 1.073 g/mL [ 13] and 1.077 g/mL [ 14], respectively, to isolate MSCs with high proliferative and differentiative potential.
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