Microsatellites were not used, as loci usable for population genetic studies are generally few and difficult to identify in Lepidoptera [ 39, 40].
The ROSA26 locus is widely used as a locus for expressing transgene sequences.
Methods for monitoring candidate loci using as little as single cell [ 37], one oocyte [ 38] or single cell blastomere [ 39] have been developed.
The number used as the locus designator indicates the order in which a particular gene or gene family member was identified and should not be confused with the systematic_locus_ID or the chromosome/linkage group on which it is found.
Yet, in other studies, ITS has been successfully used as barcode locus [10], [14], [21].
If nrITS is to be used as barcoding locus, further sequencing using cloned PCR products will be necessary to ensure that all alleles of each species are captured in the database.
This is expected to provide us with a list of loci involved in human skin pigmentation, which can be used as candidate loci for evolutionary inference.
MIP and RCB were used as marker loci to confirm whether or not a pairing was successful, because Termitomyces species lack phenotypic markers.
The vcsC2, vcsN2, vspD and vcsV2 genes were used as target loci for PCR-based amplification for the detection of the TTSS cluster in the different strains.
The introns and the cytochrome b sequences, or only the introns, were used as independent loci.
