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The yeasts Pichia pastoris and Saccharomyces cerevisiae are widely used as expression hosts for recombinant peptides.
Another important aspect is to analyse the codon usage preference among organisms used as expression systems.
For this purpose, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Yarrowia lipolytica, and Ogataea minuta have been used as expression systems.
pET28c plasmid, E. coli DH5α and E. coli BL21 (DE3) (Novagen, USA) were used as expression vector, for propagation of plasmid and as an expression host, respectively.
We present a comparison between this group of yeasts and the conventional yeasts used as expression system in view of productivity, level of secretion and quality of post-translational modifications.
However, Erwinia species (Erwinia herbicola and Erwinia citreus) that naturally accumulate 2,5-DKG from D-glucose have been used as expression host for dkr as well, and have been employed in the one-step production of 2-KLG (Anderson et al. 1985, Grindley et al. 1988, Wührer 2006 ).
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They can therefore be used as expression-based markers of drought stress and subjected to further study in the context of drought tolerance of MH96/0686 compared with susceptibility of Nyalanda.
The drought-responsive genes can now be used as expression-based markers of drought stress tolerance in cassava, and the candidate tolerance genes tested in the context of breeding (as possible quantitative trait loci) and engineering drought tolerance in transgenics.
The candidate drought tolerance genes in cassava identified in this study can be used as expression-based markers of drought tolerance in cassava or be tested in the context of breeding and engineering drought tolerance in transgenics.
Here, we describe past and recent advances in the development of several different extrachromosomal systems, discuss their retention mechanisms, and evaluate their use as expression vectors to deliver and express genomic DNA loci.
The tissue/organ with lowest expression (highest Ct) was used as calibrator (expression value = 1).
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