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RMBlast was used as a sequence search engine to list out all types of repeats.
The first 1,000 were chosen and used as a sequence data set.
This method was used as a sequence "primer", meaning that each sequence of measurements started with this column regeneration phase.
A single sample of control DNA used as a sequence reference was also found to be homozygous for the change.
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The B-F-CHPV-U1 primer used for universal probe amplification was biotinylated, and the leading strand could therefore be used as a sequencing template.
They were also sequenced and used as a sequence-quality control.
The ligated products were transformed into Escherichia coli (DH5 α) cells and the resulting plasmids were used as a sequencing template.
To improve sequencing, universal M13 primers (Promega, Sunnyvale, CA, USA) were added to the 5′ end of either forward or reverse primers and were used as a sequencing primer.
We therefore suggest that DNA from large bone crystals should be preferentially extracted, and when tSMS is used as a sequencing method, DNA templates should be prepared following DNA phosphatase treatment and using mild denaturation temperatures.
One single-copy sequence was used as a reference sequence: 8q11 SST (Single Sequence Tag), mapping at 8q11.
Thus, in the process of jackknife test, each protein sequence is used as a test sequence, and for other rounds; the sequence is included in the training dataset.
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