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A MultiClamp 700B patch-clamp amplifier was used and protocols were controlled via PC using pCLAMP10 acquisition and analysis software (Axon Instruments).
For example, the details about the instruments used and protocols followed during an experiment becomes provenance information for the data produced from that experiment.
The discrepancy between this and our data could be explicated by differences in substrains used and protocols of testing anxiety-like behaviours.
The LIVE/DEAD Viability/Cytotoxicity Kit from Molecular Probes (Eugene, OR) was used and protocols provided by the manufacturer were adopted.
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As the result, there are no widely used and tested protocols for giving a fetus anesthesia.
It is the most widely used and integrated protocol in use today around the world.
BD Mouse IFN gamma ELISPOT set (BD Bioscience, San Jose, CA) was used and standard protocol of the manual was applied.
For the MAC protocol the CSMA protocol was used, and for the RDC protocol the ContikiMAC protocol was selected.
Immunofluorescence and immunohistochemistry were performed using antibodies and protocols previously described [13], [15], [45].
Chromosomal DNA was amplified using primers and protocols previously described by Gomes et al. [6].
All data were obtained under informed consent and using procedures and protocols approved by the University of Rome Tor Vergata Intramural Ethics Committee and the EMBL Bioethics Internal Advisory Committee BIACC).
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