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Our analysis using the highest number of animal pol γ-β sequences available to date (35 sequences vs. the commonly used alignment of the human, mouse, frog, and fly sequences [ Carrodeguas et al. 2001; Fan et al. 2006; Lee et al. 2009]) argues clearly that the residues comprising the HLH-3β domain were inserted as a whole, early in the evolution of Vertebrata.
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Phylogenetic trees obtained by using alignment of the 9 concatenated spacer sequences and the neighbor-joining, maximum parsimony, and maximum likelihood methods showed similar organizations.
These candidates were further validated using alignments of the short read data sets from both other strains using Novoalign.
The approach requires sets of orthologous gene sequences, but does not require (nor use) alignments of the sequences.
Using alignments of the nuclear genomes of the four fission yeasts, we analyzed G4 motif evolutionary conservation.
Using alignments of the ORFs from a draft assembly of RM11-1a, we catalogued SNPs from genomic sequence and assessed how well these could be detected from cDNA.
To test this idea, we fit two different models for each unigene using alignments of the coding regions from cultivated tomato, Arabidopsis thaliana and S. habrochaites or S. pennellii.
The Giants used alignments of three wide receivers and one tight end on 28percentt of their offensive plays in the first three games, when Shockey was fully available.
Percent nucleotide identities were calculated using alignments of selected sequences using the software Geneious Pro v6.0.3.
REDHORSE uses alignments of NGS reads to the genome of interest as input.
10.7554/eLife.04550.006 Figure 2. Comparison of A. candida races using alignments of Illumina reads against the AcNc2 assembly.
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CEO of Professional Science Editing for Scientists @ prosciediting.com